To provide a scientific basis for clinical treatment of patients with chronic heart failure, we explored the effect of obstructive sleep apnea on laboratory indicators of patients with chronic heart failure. A total of 76 patients with chronic heart failure admitted to our hospital from January 2019 to May 2020 were selected. According to whether obstructive sleep apnea occurred, the patients were divided into normal group and observation group, with 45 cases and 31 cases respectively. The levels of inflammatory factors (TNF-α, IL-6, IL-8), cardiac function indexes (SV, LVEF, FS, CO) and vascular endothelial function (FMD, GMD) were compared between the two groups. The TNF-α, IL-6, and IL-8 levels in the normal group were 312.25 ± 26.12 ng/L, 78.62 ± 8.24 ng/L, and 139.25 ± 19.88 ng/L, while they were 428.75 ±30.23 ng/L, 151.56 ±15.69 ng/L, and 187.33 ±20.26 ng/L in the observation group. In the normal group, SV was 78.76 ± 4.27 ml/time, LVEF was 51.08 ± 3.65 %, LVFS was 27.59 ± 3.44 %, and CO was 5.72 ± 1.07 L/min. But SV was 70.58 ± 4.63 ml/time, LVEF was 46.22 ± 3.74 %, LVFS was 21.86 ± 4.24 %, CO was 4.64 ±1.02 L/min in observation group. The FMD was 9.27 ± 1.25 %, and the GMD was 22.08 ± 2.24 % in the normal group. In the observation group, the FMD was 6.73 ± 1.19 %, and the GMD was 18.87 ± 2.13 %. The differences of all above indictors were statistically significant (P<0.05). Obstructive sleep apnea in patients with chronic heart failure will aggravate the inflammatory response and affect the normal function of heart function and vascular endothelial function, which should be paid attention to in clinical practice.
The purpose of the study was to investigate the prevalence of hyperuricemia (HUA) and its correlation with cardiovascular complications in patients with type 2 diabetes mellitus (T2DM). 289 T2DM patients under the jurisdiction of the Community Healthcare Center from July 2018 to July 2019 were selected as the study subjects and divided into normal group (n=256) and abnormal group (n=33) according to the blood uric acid (UA) level. The clinical data and biochemical indexes in the two groups were compared to analyze the prevalence of HUA combined with T2DM and its correlation with cardiovascular complications. There were no significant differences in gender ratio, age, family history of T2DM, smoking and drinking history between the two groups (P > 0.05), with a significant difference in BMI (P< 0.05). The prevalence of HUA in females was significantly higher than that in males (P <0.05). The TG and TC levels in the normal group were significantly lower than those in the abnormal group (P< 0.05), and the HDL-C level was significantly higher than that in the abnormal group (P<0.05). There were no significant differences in DBP and BUN between the two groups (P > 0.05), with significant differences in FBG, SBP and Scr between the two groups (P <0.05). Logistic regression analysis showed that BMI, TG, TC, HDL-C, Scr and BUN were the risk factors affecting the occurrence of cardiovascular diseases in T2DM patients with HUA. Analysis of the ROC curve showed that cardiovascular diseases are correlated with T2DM combined with HUA, and the diagnostic efficacy of atherosclerosis is higher than that of hypertension and lower than that of hyperlipidemia. In conclusion, gender, obesity, dyslipidemia and abnormal renal function are the main factors leading to HUA in T2DM patients, while the occurrence of HUA can induce metabolic disorders and cardiovascular diseases in patients.
The purpose of the study was to investigate the clinical therapeutic effect of decitabine combined with chidamide based on coronal angiography (CAG) regimen on acute myelogenous leukemia (AML). A total of 90 AML patients admitted to our hospital from February 2016 to February 2017 were selected as the study subjects and randomly divided into control group (n=45) and experimental group (n=45). Among them, the patients in the control group were treated with decitabine combined with chidamide, while based on the treatment in the control group the patients in the experimental group received CAG regimen. After that, the clinical overall remission rate, the incidence of adverse reactions as well as the proportion of myeloblasts in bone marrow were all compared between the two groups. The clinical overall remission rate of 82.22% in the experimental group was significantly higher than that of 62.22% in the control group (P<0.05), the incidence of adverse reactions of 73.33% in the experimental group was significantly lower than that of 91.11% in the control group (P<0.05), and the proportion of myeloblasts in bone marrow in the experimental group was significantly lower than that in the control group (P<0.001). The implementation of decitabine combined with chidamide based on CAG regimen in AML patients can improve the therapeutic effect and enhance the immune function of cells, with obvious curative effect, which is worthy of application and promotion in the clinical practice.
The aim of this study is to observe the anti-oxidative stress protection and the inhibitory effect of meropenem injection on acute liver injury in septic rats. Fifty-four healthy Wistar rats were randomly divided into control group, sepsis group and drug treatment group. Sepsis models were made by cecal ligation and perforation. At 8, 16, and 24 h after the establishment of the model, the oxidative stress response of the liver, the changes of cytoinflammatory factors and the pathological changes of hepatocytes in sepsis liver injury were detected in each group. The results, compared with the control group at 8, 16 hours after cecal ligation and perforation, the oxidative stress indexes of the sepsis group and meropenem group, did not change significantly, while CRP did increase significantly (P<0.05); After 16 hours, compared with the sepsis group, MDA and TNF-α decreased, and IL-10 began to increase in the meropenem group; The meropenem group had lower serum ALT and AST ( P<0.05), liver GSH, SOD content was significantly increased (P<0.05), MDA content was reduced (P<0.05), serum CRP, TNF-α concentration was significantly reduced (P<0.01), and IL-10 significantly increased compared with the sepsis group after 24 h (P<0.01). Immunohistochemical analysis showed that meropenem can effectively reduce the oxidative stress in the liver of septic rats (P<0.05, P<0.01). Early application of meropenem injection in sepsis can resist oxidative stress response, stabilize liver cell membrane permeability, inhibit inflammatory factors, and play a certain protective effect on acute liver injury caused by sepsis.
The purpose of the study was to investigate the effect of sevoflurane combined with propofol anesthesia on the brain-derived neurotrophic factor (BDNF), S100B protein and cognitive function in elderly patients undergoing electro-prostatectomy. One hundred elderly patients undergoing electro-prostatectomy in our hospital from January 2019 to March 2020 were randomly divided into experimental group (n=50) and control group (n=50). Among them, the control group was treated with propofol anesthesia, while the experimental group was treated with sevoflurane on the basis of the control group. BDNF levels, S100 protein levels and Montreal cognitive assessment (MoCA) scores were compared between the two groups at 6 h, 24 h and 72 h before and after operation. Before operation, there were no significant differences in the three indexes between the two groups (P >0.05). At 6 h and 24 h after operation, the BDNF levels and MoCA scores in the two groups were lower than those before operation and the decrease in the experimental group was less than that in the control group (All P<0.05). The S100B protein levels were higher than those before operation, and the increase in the experimental group was less than that in the control group (All P<0.05). BDNF levels, S100B protein levels and MoCA scores in the two groups all recovered to the preoperative levels (P >0.05). Decreased cognitive function of anesthetized patients is associated with the decreased BDNF and increased S100B protein. Therefore, sevoflurane combined with propofol anesthesia can increase BDNF and decrease S100B protein, thus reducing the effect of propofol anesthesia on elderly patients undergoing electro-prostatectomy.
The aim of this study was to investigate the effect and possibility of flax lignans/solarindiglucoside (SDG) in diabetic nephropathy (DN) rats and renal tubular epithelial cells under high glucose stimulation mechanism. Experimental mice were randomly divided into control group (db/m group), diabetic nephropathy model group (db/db group) and SDG treatment group (db/db+SDG group). They were given saline or SDG (1 mg· kg-1·d-1) intraperitoneal injection for 8 weeks. At the end of the experiment, the blood glucose, 24-h urine albumin and blood creatinine levels of rats in each group were detected. TCMK-1 cells were cultured in vitro and in serum-free DMEM medium for 24 h. They were divided into control group (control), high glucose group (HG), HG+SDG group, HG+VER (VER.HSP70 specific inhibition Agent) group, HG+VER+pc-DNA-3.1 (negative control) group and HG+VER+pcDNA-3.1-TLR4 overexpression group. Real-time PCR detected TNF-α, IL- in kidney tissue and cells, IL-1β mRNA expression, Western blot analysis of TLR4 and HSP70 protein expression. Compared with the db/m group, the fasting blood glucose, 24-h urine albumin and serum creatinine levels of the db/db group were significantly increased (P<0.05). SDG can significantly reverse the above indicators in db/db mice Increased (P<0.05). The relative expression of inflammation-related factors TNF-α, IL-6, and IL-1β mRNA in db/db rats increased significantly (P<0.05). At the molecular level, we found that SDG can inhibit HSP70 protein table and reduce TLR4-mediated inflammation. SDG can slow down the TLR4-mediated inflammation in the kidney of diabetic rats by regulating the level of HSP-70 to achieve kidney protection.
NT5E is abnormally expressed in various tumors and is involved in the regulation of the occurrence and development of lung cancer (LC). The purpose of this study was to investigate the effect of overexpressing ecto-5′-nucleotidase (NT5E) on osimertinib (OSI) resistance in non-small cell lung cancer (NSCLC) PC-9 cells and its mechanism. Cell counting kit-8 (CCK-8) and colony formation assay were used to detect the sensitivity of NT5E to OSI in PC-9 cells. NT5E and epidermal growth factor receptor (EGFR) expression were detected by qRT-PCR and Western blotting. Immunohistochemistry (IHC) was used to detect the correlation between NT5E and EGFR expression in NSCLC tissues. The xenograft mouse model was established by injecting LV-NC and NT5E high-expressed PC-9 cells, and OSI intervention was given to investigate whether the high expression of NT5E would affect the inhibitory effect of OSI on tumor growth. After OSI treatment, the cell viability and colony formation of overexpressed NT5E group was significantly higher than that of the control group. It was found that NT5E overexpression could promote the expression of EGFR, and the OSI resistance induced by NT5E overexpression was reversed after the expression of EGFR was inhibited. IHC showed that NT5E and EGFR were correlated in NSCLC tissues. In vivo assays showed that NT5E overexpression facilitated tumor growth and enhanced the resistance of tumors to OSI in nude mice. Overexpression of NT5E enhanced OSI resistance of PC-9 cells, further confirming that NT5E was involved in drug resistance of NSCLC. NT5E was involved in OSI resistance in NSCLC by regulating EGFR expression.
This study aimed at observing the expression of thioredoxin domain-containing protein 5 (TXNDC5) in the epididymis of rats with varicocele (VC) and the change after high ligation of the spermatic vein. Thirty-seven-week-old male SD rats were randomly divided into sham operation, varicocele model, and treatment group. The treatment alleviated damage in the epididymis tissue of the varicocele model and inhibited the increase of miR-200c induced by varicocele. In addition, the TXNDC5 protein level in the treatment group was significantly higher than that in the model group but still lower than that in the sham operation group. In conclusion, high ligation of varicocele could repair epididymal injury, decrease miR-200c and increase TXNDC5 in the epididymis.
BACKGROUND: This study preliminarily explored the feasibility of local lymphatic system imaging and the assessment of metastatic lymph nodes using magnetic resonance lymphography (MRL) with rectal submucosal contrast injection in rectal cancer.METHODS: Eighteen patients with rectal cancer were selected and received bowel preparations. They were admitted to the Department of Colorectal Surgery, Affiliated Jinhua Central Hospital, Zhejiang University School of Medicine from September 2017 to June 2018. Contrast agents (gadopentetate dimeglumine injection) were injected under the mucosa around the tumor under colonoscopy. Magnetic Resonance (MR) imaging was used, and laparoscopic radical resection of rectal cancer was performed. The sensitivity and specificity of MRL for diagnosing lymphatic metastasis of rectal cancer were analyzed and evaluated based on the results of identification of lymph nodes in the imaging examination and the pathological examination. The patients had no adverse reaction after receiving the MRL, and MRL clearly showed the lymphatic system.RESULTS: Twenty minutes after the injection of the contrast agent, the lymph nodes began to present relatively high signal intensity, which was maintained during observation. Those lymph nodes affected by metastatic tumor presented uneven high signal intensity or no high signal intensity. After surgery, the lymph nodes affected by tumor were compared and analyzed from the perspectives of imaging and pathology. The results were herein provided: Kappa=0.870; sensitivity=83.3%; specificity=100%; Positive Predictive Value (PPV)=100%; Negative Predictive Value (NPV)=92.3%; this indicated that MRL can improve diagnostic sensitivity, and the MRL results are consistent with the pathological examination results to a large extent.CONCLUSIONS: MRL can visualize the anatomic forms and functions of lymphatic vessels and lymph nodes in the lymphatic drainage area in rectal cancer in a satisfactory way. It is safe and feasible for diagnosing lymphatic metastasis of rectal cancer. However, further large-scale and in-depth clinical studies are still necessary for demonstration.
The purpose of the study was to investigate the small intestinal bacterial growth and its correlation with spontaneous peritonitis, liver function and disease severity in patients with post-hepatitis B cirrhosis (PHBC). A total of 150 PHBC patients admitted to our hospital between October 2017 and June 2019 were randomly selected. Among them, 30 PHBC patients complicated with spontaneous peritonitis were selected as spontaneous peritonitis group and 30 PHBC patients complicated with liver dysfunction as liver dysfunction group. Besides, 90 PHBC patients were divided into groups A, B and C (each group with 30 cases) as liver cirrhosis was classified as grades A, B and C according to Child-Pugh classification, and another 30 healthy adults with normal health examination results over the same period were selected as control group. All patients received lactulose hydrogen breath test to detect small intestinal bacterial growth and analyze the occurrence of small intestinal bacterial overgrowth; the small intestinal bacterial growth was compared between the control group and other groups and the area under curve (AUC) of ROC curve in each group was calculated and compared. Meanwhile, the small intestinal bacterial growth in groups A, B and C was compared to analyze the correlation between small intestinal bacterial growth and the PHBC severity of patients. The number of patients with small intestinal overgrowth and the AUC in the spontaneous peritonitis group, the liver dysfunction group and groups A, B and C were significantly higher than those in the control group, with statistical significance (P<0.05); additionally, as small intestinal bacterial growth varies with different severity of liver cirrhosis, the small intestinal bacterial overgrowth in group B was higher than that in group A and lower than that in group C, with statistical significance (P<0.05). The ROC curve showed that the diagnostic efficiency of spontaneous peritonitis for intestinal bacterial growth was higher than that of liver function and lower than that of disease severity. In conclusion, there is a close correlation between the small intestinal bacterial overgrowth and spontaneous peritonitis, liver function, and disease severity in PHBC patients.