J Biol Regul Homeost Agents. 2020 Apr 24;34(2). doi: 10.23812/20-36A. [Epub ahead of print]
MicroRNA-206 inhibits tumor metastasis of nasopharyngeal carcinoma through targeting G6PD.
- 1 Department of Ear-nose-throat (ENT), Yidu Central Hospital of Weifang, Qingzhou, Weifang, Shandong, China.
- 2 Department of Pathology, Zibo Central Hospital, Zhangdian, Zibo, Shandong, China.
- # Contributed equally
Nasopharyngeal carcinoma (NPC) is a main type of otolaryngological malignancy. In many cancers, miR-206 functions as a tumor suppressor, suppressing cell proliferation, migration and invasion. The purpose of this study was to explore how miR-206 worked on cell metastasis in NPC. The mRNA levels of miR-206 and G6PD were determined in NPC tissues and cell lines by RT-qPCR and Western blot. Transwell assay was applied to evaluate the migratory and invasive capacities. Dual luciferase reporter assay was employed to confirm that miR-206 mediated the expression of G6PD in C666-1 cells. In this study, miR- 206 was downregulated, whereas G6PD was upregulated in NPC tissues and cell lines. In addition, G6PD was identified as a direct target gene of miR-206 in C666-1 cells. The expression of G6PD was mediated by miR-206, which could partially reverse the inhibitory effects of miR-206 on the migration, invasion and EMT in C666-1 cells. In conclusion, miR-206 regulated the migratory, invasive and EMT abilities through directly targeting the 3′-UTR of G6PD mRNA in C666-1 cells. The newly identified miR-206/G6PD axis provides novel insight into the pathogenesis of nasopharyngeal carcinoma.
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EMT, G6PD, invasion, miR-206, nasopharyngeal carcinoma
- Journal Article