J Biol Regul Homeost Agents. 2019 September-October,;33(5):1387-1394.


Effect of RhoC silencing on multiple myeloma xenografts and angiogenesis in nude mice.

Liu K#1, Sun MM#2, Zhao ZH1, Wei N1, Jiang GZ1, Wang ZY1, Zhang L1, Zhu XY3, Dai LP4, Yang HM5, Wang T1, Chen KS1.

Author information

1 Department of Pathology, The First Affiliated Hospital of Zhengzhou University, Henan Key Laboratory of Tumor Pathology, Zhengzhou, China.
2 Department of Urinary Surgery, Harbin Medical University Cancer Hospital, Harbin, China.
3 Histology and Embryology Teaching and Research Center, School of Basic Medical Sciences, Zhengzhou University, Zhengzhou, China.
4 Henan Academy of Medical and Pharmaceutical Sciences Department of Epidemmiology, Zhengzhou, China.
5 Henan Medical College Basic Medical Department, Zhengzhou, China.
# Contributed equally

Abstract

In this study, we investigated the expression of RhoC in the multiple myeloma (MM) cell line RPMI- 8226, as well as the effects of silencing RhoC on the growth of tumor xenografts and tumor-induced angiogenesis in nude mice with MM. For this purpose, we transduced RPMI-8226 cells with lentiviral particles overexpressing short hairpin RNAs (shRNA) targeting RhoC. Tumor xenografts were generated by subcutaneously injecting nude mice with RPMI-8226 cells overexpressing control shRNA [negative control (NC) group] or the RhoC shRNA [the experimental (S) group], respectively. RhoC protein and mRNA levels in the tumor xenografts were measured. Nude mice were also subcutaneously inoculated with Matrigel mixed with vascular endothelial growth factor, and CD31 and KI67 levels in the tumor xenografts were measured by immunohistochemistry. Similarly, we assessed tumor xenograft growth and angiogenesis in Matrigel implants in the mice of both groups. We found that RhoC levels, microvessel density, and CD31 labeling index were more reduced in the S group than in the NC group. However, there was no significant difference in the size of tumor xenografts between the 2 groups. The number of new vessels and the neovascular length in the Matrigel implants were significantly lower in the S group than in the NC group. Therefore, we concluded that RhoC expression in myeloma xenografts has important effects on the induction of angiogenesis.

KEYWORDS:

RhoC, blood vessels, matrigel implants, multiple myeloma, xenografts

Publication type

  • Journal Article

You may also like...