Effect of polydatin on expression of p53 and Notch1 in brain tissue of ischemic cerebrovascular disease.
F.Y. Chen 1, X.Y. Fang 2, H. Zhang 3
1 Neurosurgery, Ningbo Second Hospital, Ningbo City, Zhejiang Province, China
2 Facial Features, Yinzhou Second Hospital of Ningbo, Ningbo City, Zhejiang Province, China
3 Neurosurgery, The Second Affiliated hospital of Zhejiang University School of Medicine, Hangzhou City, Zhejiang Province, China
Secondary injury of brain tissue following cerebral infarction exhibits a complicated pathogenesis that is attributed to the induction of apoptosis and inflammatory response. The present study aimed to investigate the polydatin neuroprotective effects and their mode of action in cerebral ischemic injury. A total of 80 healthy adult male Sprague-Dawley rats were randomly divided into a Sham operated group, a permanent middle cerebral artery occlusion (MCAO) group, a Polydatin low dose group and a Polydatin high dose group. A total of 20 rats were used in each group. The right MCAO model of rats was established using the modified Longa suture method. The animals in the intervention group were injected intraperitoneally with 12.5mg/kg and/or 50mg/kg of polydatin following infarction. The expression levels of p53 and Notch 1 were measured by immunohistochemical techniques. The degree of left limb hemiplegia in the MCAO group was different compared with that of the low and high dose group of polydatin. The score for the neurological function in the high-dose group of polydatin was lower than that in the MCAO group (High vs MCAO: 2.4±0.31 vs 3.9±0.23, P < 0.05). The results indicated that high dose polydatin could reduce brain edema following cerebral infarction and improve the behavioural score in rats.