Genetic diversity of mycobacterium tuberculosis in northern Greece.
G. KAZDAGLIS 1*, B. MOLINA-MOYA 2*, K. MANIKA 3, P. IOANNIDIS 4, D. PAPAVENTSIS 4, E. VOGIATZAKIS 4, M. PANOPOULOU 5, A. MELIDOU 1, J. DOMÍNGUEZ 2**, N. MALISIOVAS 1** and G. GIOULA 1**
*These authors contributed equally to this work ** Co-senior authors
1 Microbiology Department, Medical School, Aristotle University of Thessaloniki, Greece
2 Microbiology Laboratory, University Hospital Germans Trias i Pujol, Institute of Investigation Germans Trias i Pujol, Badalona, Autonomous University of Barcelona, CIBERES, Spain
3 Pulmonary Department, Aristotle University of Thessaloniki, G. Papanikolaou Hospital, Greece
4 Microbiology Laboratory and National Reference Center for TB, Sotiria Chest Diseases Hospital, Athens, Greece
5 Microbiology Department, Medical School, Democritus University of Thrace, Greece
The aim of the present study was to describe the genetic diversity of Mycobacterium tuberculosis (M.tuberculosis) strains circulating in the region of Northern Greece. A total of thirty-seven M. tuberculosis clinical isolates were analysed by the spoligotyping method. According to the results, six clusters comprising seventeen strains were detected, and the remaining twenty strains showed unique patterns. The M.tuberculosis families according to SITVITWEB were distributed as follows: Haarlem (H) (27.0%); T (24.3%); Beijing (13.5%); Latin-America and Mediterranean (LAM) (5.4%) and S (2.7%). The remaining isolates (27%) did not match any isolates within the database and they were characterized as orphans. Regarding GenoType MTBDRplus results, two strains (5.4%) were Multi-Drug-Resistant, four strains (10.8%), were isoniazid monoresistant, while the remaining thirty-one strains (83.8%) were susceptible. In conclusion, in the region of Macedonia-Thrace (Northern Greece), there was high phylogenetic diversity among M. tuberculosis isolates. Molecular tools used and data presented can have regional and national impact on tuberculosis control.